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Cre1 gene

WebApr 26, 2002 · Vectors pACEC31 and pACEC41 carrying full-length copies of thecre1 gene harboring the mutations S241A and E244V, respectively, and the hph transformation marker were constructed as follows: the respective mutations were first introduced by PCR into a 250-bp cre1 fragment, using CreS241A-f and CreE244V-f, respectively, as the respective … WebMar 31, 2015 · In M. thermophila, the CRE-1 ortholog MtCRE1 is a key repressor of cellulase gene expression, and either the silencing or deletion of Mtcre1 effectively improves cellulase production (15, 44).

Identification of CRE1 as a cytokinin receptor from - Nature

WebNov 27, 2007 · The promoter of the cre1 gene, encoding the glucose-dependent regulator CRE1 from the β-lactam producer Acremonium chrysogenum, carries 15 putative CRE1 … WebFeb 22, 2001 · CRE1 expression conferred a cytokinin-dependent growth phenotype on a yeast mutant that lacked the endogenous histidine kinase SLN1 (ref. 10), providing direct … honda goldwing upgrades https://elyondigital.com

Cloning of an intronless cre1 gene from Chaetomium thermophilum

WebMoreover, complementation of nodulation-defective mutants chk1/cre1 with Arabidopsis AHK4/CRE1 gene rescued nodule initiation deficiency, but the AHK4 gene from Arabidopsis was not able to complement nitrogen fixation deficiency (Boivin et al. 2016), suggesting that cytokinin perception’s importance in the late stages of nodule development ... WebDec 10, 1996 · Mycoparasitic interaction relieves binding of the Cre1 carbon catabolite repressor protein to promoter sequences of the ech42 (endochitinase-encoding) gene in … WebJul 1, 2001 · Root growth in cre1 also displayed increased resistance to inhibition by cytokinin. CRE1 mapped to the top of chromosome 2, and a histidine kinase gene located in the region was identified as a candidate and shown to complement cre1 mutants following transformation. One predicted form of CRE1 is a 1057 amino acid protein; a second form … honda goldwing used bikes for sale

Cloning of an intronless cre1 gene from Chaetomium thermophilum

Category:A CRE1- regulated cluster is responsible for light dependent …

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Cre1 gene

Factors regulating cellulolytic gene expression in filamentous …

WebOct 10, 2014 · Carbon catabolite repression by CRE1 (or its CreA orthologs in other filamentous fungal species), a member of the C 2 H 2 class of DNA-binding proteins that recognizes a 5′SYGGRG-3′ consensus sequence (Felenbok et al ., 2001 ), is the major negative regulator of cellulase and hemicellulase gene expression because it inhibits … WebProtein CREG1 (Cellular Repressor of E1A-stimulated Genes 1) is a protein that in humans is encoded by the CREG1 gene. [5] [6] The adenovirus E1A protein both activates and …

Cre1 gene

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WebJun 18, 1999 · The cre1 gene expression in the transformants was confirmed with the detection of CRE1 amongst intracellular yeast proteins analyzed by SDS-PAGE and Western blot using antibodies raised against the zinc finger domain (not shown). WebAug 15, 2024 · Additionally, gene regulation by CRE1 is specific for light and darkness with surprisingly few genes being regulated in light as well as darkness . Genes regulated by CRE1 are clustered in the genome Evaluation of the genomic loci of genes up- or down-regulated by CRE1 in light and darkness revealed a non random distribution of 259 …

WebAug 20, 2024 · Additional file 1: Figure S1. Deletion of cre1-96 in T. reesei Rut-C30. (A) Rut-C30 was transformed with the plasmid pMS*-5hph3cre1 that bears the deletion cassette consisting of the hygromycin resistance gene under the pki promoter and the terminator of cbh2 (dark grey arrow, hph) to replace the native cre1-96 gene (light grey arrow, cre1 … WebMar 25, 2024 · To tag Cre1 with 5×myc, the strain Cre1:5myc was constructed by inserting a sequence encoding 5×myc tag at the native genomic locus of the cre1 gene in the WT strain. Mrcre1 open reading frame without a stop codon (as a 5′ homologous arm) and its 3′ flanking region were PCR amplified from ARSEF 2575 genomic DNA and then subcloned …

WebMar 21, 2024 · Entrez Gene Summary for SOX9CRE1 Gene. This region represents an enhancer that is thought to regulate expression of the SRY-box 9 (SOX9) gene, located … WebMar 21, 2024 · CRE1 is a RefSeq non-genic functional element . Its equivalent regulatory element (s) in GeneHancer is GH05J088388 GH05J088388 targets: piR-30832-008, CM034955-246, ENSG00000271904, TMEM161B, TMEM161B-DT, MEF2C Genomic …

WebApr 9, 2024 · Prg4 also encodes the related gene products superficial zone protein (SZP), megakaryocyte-stimulating factor (MSF), and hemangiopoietin ... (TSS) of Prg4 (i.e., CRE1, at −4503 base pair [bp] relative to the TSS) or in the first intron of this gene (i.e., CRE2, at +679 bp relative to the TSS) . In addition, we found that FFSS could ...

WebCre1 is an important transcription factor that regulates carbon catabolite repression (CCR) and is widely conserved across fungi. This gene has been extensively studied in several Ascomycota species, whereas its role in gene expression regulation in the Basidiomycota remains poorly understood. history of maori tattoosWebMar 21, 2024 · GeneCards Summary for SOX9CRE1 Gene SOX9CRE1 (SOX9 Enhancer Cre1) is a Functional Element gene. Diseases associated with SOX9CRE1 include Campomelic Dysplasia . Additional gene information for SOX9CRE1 Gene NCBI Entrez Gene (108021841) Search for SOX9CRE1 at DataMed Search for SOX9CRE1 at … history of mapungubwe pdfWebJun 9, 2024 · Summary: Cre1 is an important transcription factor that regulates carbon catabolite repression (CCR) and is widely conserved across fungi. This gene has been extensively studied in several Ascomycota species, whereas its role in gene expression regulation in the Basidiomycota remains poorly understood. Here, we identified and … honda goldwing used trikesWeb1 day ago · CRE1, a conserved carbon catabolite repressor in fungi, is the first demonstrated transcription factor in the biocontrol with T. harzianum. Before contact with the plant pathogen, CRE1 can bind to two single sites in the promoter of chitinase gene chit42 to inactivate its expression. honda goldwing vs harleyWebDec 10, 1996 · Mycoparasitic interaction relieves binding of the Cre1 carbon catabolite repressor protein to promoter sequences of the ech42 (endochitinase-encoding) gene in Trichoderma harzianum. Matteo Lorito, ... This gene was chosen as a model because its expression is triggered during mycoparasitic interaction [Carsolio, C., Gutierrez, A., … honda goldwing vs bmw k1600 grand americaWebAug 20, 2024 · Deletion and constitutive expression of cre1-96 in T. reesei Rut-C30. To identify the function of Cre1-96 in Rut-C30, the encoding gene was deleted from the genome. Therefore, a deletion cassette was integrated by homologous recombination at the cre1-96 locus, resulting in a gene replacement of cre1-96 in Rut-C30. Two cre1-96 … honda goldwing vs harley ultraWebThe PCR product was ligated into pTZ57R/T vector and transformed in E.coli TOP 10 strain. cDNA from total RNA was utilized as template for RT-PCR analysis, which confirmed the presence of an intronless cre1 gene in the model organism. This is the first report on isolation ofcre1 gene fragment in C.thermophilum. history of maple tapping