Hitrap heparin hp manual
WebHiTrap™ SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample … WebMaximum flow rates1 HiTrap 1 ml: 4 ml/min, HiTrap 5 ml: 20 ml/min Recommended flow rates HiTrap 1 ml: 1 ml/min, HiTrap 5 ml: 5 ml/min Chemical stability All commonly used …
Hitrap heparin hp manual
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WebHiTrapQ HP 使用手册. 选择合适的上样环,例如 5ml loop. 用纯水清洗 loop 三次,再用 buffer A 清洗 loop 三次,赶走气泡;同时清洗上样口。. 将样品置于上样口,用针筒抽吸样品进入 loop。. (切忌吸入气泡). File--设定的程序--show detail--确认无误--START。. 特别注 … WebHisTrap™HP is a ready to use column, prepacked with precharged Ni Sepharose™ High Performance. This prepacked column is ideal for preparative purification of Histidine …
WebThis page shows how to use heparin in the separation of DNA binding proteins used in HiTrap Heparin HP, HiPrep 16/10 Heparin FF and Heparin Sepharose 6 Fast Flow products ... Sweden) using HiTrap Heparin HP, 5 mL. Figure 4. scCro8 purification on HiPrep 16/10 Heparin FF. Performing a Separation. Binding buffers: 20 mM Tris-HCl, pH … WebHisTrap HP, 1 x 5 ml. High-performance immobilized metal affinity chromatography (IMAC) columns for His-tag recombinant protein purification. Change Product.
WebProduct Support. HiTrap Q FF is a strong anion exchange chromatography column for small-scale protein purification. Packed with Q Sepharose Fast Flow strong anion exchange resin. Convenient and affordable for fast ion exchange separations, either alone or connected in series. High flow rates and good scale-up potential. Web280 = 14 was coupled to a 1 ml HiTrap NHS-activated HP column. After completed coupling, the coupling solution was washed out from the column with 3 ml of coupling buffer (e.g. 0.2 M NaHCO 3, 0.5 M NaCl, pH 8.3). When 0.5 ml of this solution was separated on the PD-10 Desalting
WebHiTrap ® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap ® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut.
WebHiTrap Butyl FF, HiTrap Butyl-S FF, HiTrap Butyl HP, and HiTrap Octyl FF are prepacked 1 mL and 5 mL, ready-to-use hydrophobic interaction chromatography (HIC) columns. … finance tripura treasuryWebHeparin binds to a wide range of biomolecules via both affinity and ion exchange mechanisms.Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma proteins.Simple operation with a syringe, a pump or a chromatography system such as ÄKTA designStable over pH 5 to 10 and tolerates all … finance translation for spanishWebDescription. HiTrap Heparin HP columns are prepacked with Heparin Sepharose High Performance for high-resolution purification of proteins with affnity for heparin such as … finance t rocWebThe HiPrep Heparin FF 16/10 is intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes. Product No. per pack Code No. HiPrep Heparin FF 16/10 1 x 20 ml 28-9365-49 HiTrap™ Heparin HP 5 x 1 ml 17-0406-01 HiTrap Heparin HP 1 x 5 ml 17-0407-01 HiTrap Heparin HP 5 x 5 ml 17-0407-03 finance triple crownWebユーザーズマニュアル GE SP HP の仕様概要. ページ 1 GE Healthcare Instructions 71-7149-00 AN HiTrap ion exchange columns HiTrap SP HP, 1 ml and 5 ml HiTrap Q HP,... ページ 2 Code No. Designation No. supplied 17-1151-01 HiTrap SP HP 5 x 1 ml 17-1152-01 HiTrap SP HP 5 x... ページ 3 1. finance treadmill with bad creditWebHiTrap™ Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different … gsp airport master planWeb71-7149-00 AP 5 Table 2. HiTrap SP HP and HiTrap Q HP columns characteristics 1 Water at room temperature 2 The ranges given are estimates based on our knowledge and experience. Please note the following: pH stability, long term refers to the pH interval where the medium is stable over a long period of time without adverse effects on its subsequent … gsp airport customer service