Nettet1. Hoechst 33342 Staining solution (HO) 2. Hoechst 33342 1.0 mg/ml in dH2O 3. DiOC5 (Molecular Probes) 1.0mg/ml in DMSO . Staining . 1. HO is added to cells in culture … NettetWith the addition of Hoechst 33342, a specific DNA fluorochrome, we can correlate apoptotic cell death with cell cycle analysis. Unlike other DNA dyes, Hoechst 33342 crosses the cellular membrane and stains specific A-T regions of the DNA, yielding DNA content information.
Multiparametric Cell Cycle Analysis Using the Operetta High
NettetNuclear counterstain for fluorescence microscopy Invitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. NettetHoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. These dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains. Biotium offers Hoechst 33258 and Hoechst 33342 dyes … brotherhood mark 8 36
Cell Cycle Analysis, Flow Cytometry Core Facility
NettetHoechst 33258 nucleic acid stain is a popular nuclear counterstain that emits blue fluorescence when bound to dsDNA. Its uses are similar to Hoechst 33342 (H-1399) for counterstaining, apoptosis and cell cycle … Nettet26. nov. 2012 · The Hoechst 33342 dye is similar to DAPI in that both are UV-excited, minor groove-binding, and emit signals proportional to total DNA content. Both are maximally excited around 355 nm and emit around 460 nm. A UV light source is required, which may harm the cell. Nettet1. sep. 2016 · Hoechst 33342 is a nucleic acid dye that emits blue florescence when bound to DNA. Hoechst 33342 signal is changed between different phases of cell cycle due to the amount of DNA. Hoechst 33342 is strongest during interphase when DNA synthesis takes place. Ki-67 is a cellular marker for proliferation. cargo green pants outfit men