2024 Mouse hepatic stellate cell line

Mouse hepatic stellate cell line

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August undefined, 2024

NettetHepatic stellate cells (HSCs) are the primary extracellular matrix-producing cells within the liver and have numerous vital functions. A robust protocol for the isolation and … Nettet12. okt. 2024 · Chu, P. S. et al. C-C motif chemokine receptor 9 positive macrophages activate hepatic stellate cells and promote liver fibrosis in mice. Hepatology 58 , 337–350 (2013). CAS PubMed Google Scholar

List of commonly used liver cell lines. - ResearchGate

Nettet15. aug. 2005 · Activated pancreatic stellate cells (PSC) play a central role in this process. However, analysis of the molecular mechanisms leading to PSC activation is hampered by the lack of an established... Nettet4. okt. 2024 · Mouse hepatocyte cell line AML12 was purchased from the Chinese Academy of Sciences (Shanghai, China) and cultured in AML12 specific culture medium (Procell, Wuhan, China). Mouse hepatic stellate cell (CP-M041) was purchased from Procell (Wuhan, China) and maintained in specific hepatic stellate cell medium (CM … cloppenburg copy shop

Human Pancreatic Stellate Cells - ScienCell Research Laboratories

Nettet19. jun. 2024 · Hepatic stellate cell line LX-2 were obtained from the Cell Center of Shanghai Institutes for Biological Sciences. Although, the study of stellate cell … Nettet3. apr. 2024 · Materials and methods. ICR mice with CCl 4-induced hepatic fibrosis were supplemented with Sch B (40 mg/kg) for 30 d and LX2 cells were treated with Sch B (5, 10 and 20 μM) for 24 h.Cellular senescence was assessed by senescence-related indicators senescence-associated β-galactosidase (SA-β-gal) activity and the expression of p16, … NettetLX-2 Human Hepatic Stellate Cell Line, The LX-2 human hepatic stellate cell line has been extensively characterized and retain key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them a highly suitable model of human hepatic fibrosis. Expand SCC066 bodybuilder\\u0027s cy

Eupatilin Ameliorates Hepatic Fibrosis and Hepatic Stellate Cell ...

Ideal surface markers to identify hepatic stellate cells?

Nettet12. sep. 2024 · We previously demonstrated that activated LX-2 can stimulate the transformation of M1 to M2 macrophages in hepatic stellate cell lines, but it is unknown whether primary cell lines have the same function. So, we isolated and cultured HSCs from mice and used TGF-β1 to activate them. Nettet19. apr. 2024 · Hepatic stellate cells (HSCs) are specialized pericyte-like cells, located in the space of Disse, that wrap liver sinusoids [ 1] and interact with both hepatocytes and liver sinusoidal endothelial cells [ 2 … bodybuilder\u0027s cyNettetHepatic fibrosis is characterized by the abnormal deposition of extracellular matrix (ECM) proteins. During hepatic fibrogenesis, hepatic stellate cell (HSC) activation followed … bodybuilder\u0027s fc

"Nettet11. mai 2024 · We first transfected Hepa 1–6 cells, a mouse hepatoma cell line, ... Hepatic stellate cells (HSCs) and Kupffer cells (KCs) were isolated according to the published protocol 29. " - Mouse hepatic stellate cell line

Mouse hepatic stellate cell line

Stellate Cell Line - an overview ScienceDirect Topics

Nettet19. apr. 2024 · To obtain meaningful results of hepatic stellate cell (HSC) function, it is crucial to use highly pure HSC populations. Our aim was to optimize HSC isolation from mice livers without exploiting the characteristically transient vitamin A autofluorescence of HSC. HSCs were isolated from C57BL/6 mice using a two-step collagenase digestion … NettetThese mouse hepatic stellate cell (HSC) lines express WT TLR4 or TLR4 single nucleotide polymorphisms (SNP) variants, D299G or T399I. Hepatic stellate cells …

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NettetMouse Hepatic Stellate Cells (MHSteC) provided by Innoprot are isolated from swiss mouse liver tissue. MHSteC are cryopreserved immedaitelly after isolation and delivered frozen. Each vial of Mouse Hepatic … NettetGreen tea polyphenols have been shown to protect against experimental alcohol-induced hepatic fibrosis in rats. 97 As an antioxidant, tea polyphenols show antifibrosis effects observed by Masson staining in rats with alcoholic liver disease, and the effect may be positively correlated with the dose.

NettetActivated hepatic stellate cells are a major cellular source of MCP-1 in injured livers . In line with this, the expression of α-smooth muscle actin ( α-sma ), a marker for HSC … Nettet26. mai 2024 · Primary hepatic stellate cells from two mice of the same group were pooled as one sample. The purity of primary HSCs was determined by adding Oil Red O staining to freshly isolated primary cells that had been cultured for 2 days (>97%). 24 HSC viability was determined using the Trypan blue exclusion test (>90%).

NettetNormal mouse hepatocyte cell line FL83B (ATCC CRL-2390) was cultured in Ham’s F-12 K medium containing 10% fetal bovine serum ... liver fibrosis. Since HSCs are major sources of fibrogenic liver cells, it was expected that Daxx would be expressed in hepatic stellate cells; however, the IHC data indicated that Daxx was mainly expressed in ... NettetMethods: Mouse LPCs were co-cultured in Transwell plates with an immortalized human hepatic stellate cell line (HSC-Li) we previously established. The morphology, …

Nettet23. nov. 2024 · Fate-Mapping Evidence That Hepatic Stellate Cells Are Epithelial Progenitors in Adult Mouse Livers. Stem Cells. 26, 2104–2113 (2008). Article CAS PubMed PubMed Central Google Scholar DeLeve, L ...

Nettet29. mai 2024 · Immortalized hepatic stellate cells (HSCs) established from mouse, rat, and humans are valuable in vitro models for the biomedical investigation of liver … bodybuilder\u0027s goalNettet30. apr. 2024 · We searched through the PubMed database with the keywords “liver fibrosis”, “hepatic stellate cells”, and “herbal medicine”. We aim to highlight the role of herbal drugs in modern medicine and provide insights and perspectives on the research and development of first-line fibrosis therapies targeting hepatic stellate cells. cloppenburg facebookNettet10. mai 2024 · Activation of hepatic stellate cells (HSCs) in liver injury is the primary driver of hepatic fibrosis. In this Review, Tsuchida and Friedman detail the varied … cloppenburg familyNettetActivated hepatic stellate cells are a major cellular source of MCP-1 in injured livers . In line with this, the expression of α-smooth muscle actin ( α-sma ), a marker for HSC activation, was upregulated in response to BDL, with significantly higher expression levels in the Fhl2 -deficient mice than the wt mice ( Figure 2 A). cloppenburg fahrradNettetC57BL/6 Mouse Primary Hepatic Stellate Cells from Cell Biologics are isolated from liver tissues of pathogen-free laboratory adult mice. Cells are grown in tissue culture flasks … bodybuilder\\u0027s goal crossword clueNettetAML12. CRL-2254 ™. AML12 (alpha mouse liver 12) cells are hepatocytes isolated from the normal liver of a 3-month-old mouse. Use these cells as a transfection host or in … cloppenburg derby cycleNettet11. mai 2024 · We first transfected Hepa 1–6 cells, a mouse hepatoma cell line, ... Hepatic stellate cells (HSCs) and Kupffer cells (KCs) were isolated according to the … bodybuilder\u0027s goal crossword