Psin-ef2 vector
WebJul 18, 2024 · The full length of CTBP1-DT was generated by subcloning the PCR-amplified human CTBP1-DT sequence and cloned into pSin-EF2-vector. The DDUP ORF with FLAG-tag expression plasmid was generated by cloning the full length of CTBP1-DT. WebPsin Ef2 Puromycin Lentiviral Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article …
Psin-ef2 vector
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WebSystemic delivery of pSin-EF2-ELMO1-Pur facilitates continuous ELMO1 expression in colonic tissues. [A] Immunohistochemical staining of ELMO1 in the colons of untreated mice or mice treated with... WebThe pSin-EF2- GFP-Puro (EF1 a promoter) provided maximal GFP expression in both the undifferentiated cells as well as the day 10 differentiated cells with greater than 95% of …
http://www.labbase.net/Supply/SupplyItems-4229347.html WebMay 11, 2024 · abstract type AbstractPolynomial end """ Polynomial <: AbstractPolynomial Polynomials written in the canonical form --- Polynomial(v::T) where T<:Union{Vector{<:Real}, NTuple{<:Any, <:Real}}) Construct a `Polynomial` from the list of the coefficients. The coefficients are assumed to go from power 0 in the ascending order.
WebJun 1, 2024 · The lentiviral expressing vector encoding human Oct4 (pSin-EF2-Oct4-Pur, Addgene plasmid 16579) was obtained from Addgene (Cambridge, MA) . The lentiviral control vector pSin-null was derived from pSin-EF2-Oct4-Pur as previously described . For knockdown experiments, pLKO.1-puro-based lentiviral vectors, including stem-loop … WebApr 20, 2012 · Fig. 7 outlines the vector (pSin-EF2-TK-Puro), with TK driven by the EF1α promoter. hES H9 cells were treated with BMP4 , allowing for trophoblast differentiation. Cells were infected with either the SSEA4 or CD24 antibody conjugated m 168 pseudotyped virus bearing the pSin-EF2-TK-Puro lentiviral vector, five days post BMP4 addition and ...
WebNov 17, 2024 · GFP-human Med15 and TagRFP-human DYRK3 fusion protein were subcloned into the lentiviral expression vector pSin-EF2 by ClonExpress II One Step Cloning Kit (Vazyme Biotech, C112). Primers used to clone lentiviral vectors are provided in Additional file 6: Table S3. The human embryonic kidney 293T cell line was used as a host …
WebAddgene inc psin ef2 modulation vector Psin Ef2 Modulation Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. … boots plastic bottlesWebThe empty pSin-EF2 vector (lenti-vector) was used as a control. Observation of morphologic changes. The cells were seeded into six-well culture (Corning Incorporated, Corning, NY, USA) plates and transferred with miR-16 or miR-Ctrl. After incubation for 24 hours, the cellular morphology was observed by phase contrast microscope (Olympus America ... hat on harry potterWebApr 6, 2024 · Briefly, the pSin-EF2-TIPE3-HA or empty vector, as well as the lentivirus packaging plasmids psPAX2 and pMD2.G, were co-transfected into 293FT cells using the calcium phosphate method. After... boots plastic dental picksWebSep 14, 2024 · The empty vector pSin-EF2-Pur was used as a control. The plasmids were verified by sequencing. For stable knockdown of ELMO1, GV248-puro [Shanghai Genechem Co., Shanghai, China] containing hairpin sequence targeting ELMO1 or a control plasmid [control hairpin] was co-transfected with psPAX2 and pMD2G [Addgene, Cambridge, MA, … hat on hook vectorWebConstructs expressing eGFP from the pSin- EF2-GFP-Puro based vector were used in subsequent studies. A key bottleneck in many stem cell applications is the ability to identify, select or ... boots plasticWebOct 5, 2024 · To determine that whether LASP1 is a direct target of miR-203a-3p, CNE-2 and SUNE-1 cells were co-transfected with miR-203a-3p mimic or miR-Ctrl (50 nM, RiboBio) and the pSin-EF2-puro-LASP1 (LASP1) or empty pSin-EF2-puro-Vector (Vector) (2 μg; Addgene, Cambridge, MA, USA). The cells were harvested for assays 48hs after transfection. boots plastic recycling schemeWebApr 6, 2024 · The pSin-EF2-puro-Vector plasmid was used as negtive control. HNSCC cells stably overexpressed TIPE3 were constructed as we previously reported [17, 19]. Briefly, the pSin-EF2-TIPE3-HA or empty vector, as well as the lentivirus packaging plasmids psPAX2 and pMD2.G, were co-transfected into 293FT cells using the calcium phosphate method. … boots plastic pants