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Web1 day ago · Given the direct involvement of Hla in upregulating ADAM10 activity and S. aureus-induced necroptosis, ... THP-1 cells infected with S. aureus at an MOI of 20 for 6 h with different treatments were washed with PBS and lysed using T-PER Tissue Protein Extraction Reagents followed by centrifugation at 10 000g for 8 min. Web3.1.2.1 Labeling effector cells and target cells. 1. Count THP-1 cells. If cell number allows, duplicate or triplicate wells are set up for each condition. 2. Count the target cells. Take at … WebAug 4, 2024 · We co-cultured human hepatoma HepG2 cells with M1 or M2 derived from human monocytic leukemia THP-1 cells to examine the roles of M1 and M2 in DIC-induced cytotoxicity. DIC was added to the direct or indirect co-cultures of HepG2 cells with M1 or M2 (HepG2/M1 or HepG2/M2, respectively) at cell ratios of (1:0, 1:0.1, 1:0.4, and 1:1). heart shaped yoga pants